(b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. ®Molecular Devices SpectraMax 190 Microplate Reader. D. The dichloromethane, methanol, and hexane extracts were eluted four times and the absorbance spectra of the samples was measured at a range of wavelengths from 200 to 750 nm. The absorbance was determined at 450 nm with SpectraMAX-190 ELISA reader (Molecular Devices, Sunnyvale, CA). 00 . Seller InformationAnytimeLabTrader. Total flavonoid content The total flavonoid content of the extracts was determined according to the method described by Herald et al. D) units. 7. Page 1Method 66DNA Analysis on the Molecular Devices Spectramax Plus and Spectramax 190 microplate readers Quick Reference Guide Section A: Plate Materials A UV transparent microplate is required for. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 0 nm full width half maximum. 316 grating motor driver faulty. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Small volumes (5 μL, 50 mM) of compound solution, dissolved into DMSO, were added to the. We used three technical replications for each biological replication and measured absorbance at 595 nm using a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). Molecular Devices SpectraMAX 190 Microplate Reader/Spectrophotometer, Tested. 3 mmol) was added to a flask containing a 10 mL of methanol solution of HPPH (0. 5mm filters. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- StakMax Handling Stacker Base Unit. Widen the range of quantitative experiments with a 190-1000 nm wavelength range. 8 in. (SpectraMax ® 190, Molecular Devices). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. 1. Note: Due to. The SPECTRAmax 190 can accommodate standard 96-well microplates, strip wells, and Þlter-bottom microplates. Plasma biomarker levels were measured by enzyme-linked immunosorbent assay (ELISA; R&D unless otherwise stated) using a SpectraMax 190 plate reader (SpectraMax 190 microplate reader, RRID : SCR_018932). For customers outside the U. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 0 nm. Upgradeable modules are also available including western blot, cell imaging, and fast. DNA provides the same sensitivity as a spectrophotometer. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. The VersaMax and SpectraMax 190 read 96-well microplates. DNA provides the same sensitivity as a spectrophotometer. We have 1 Molecular Devices SpectraMax 190 manual available for free PDF download: User Manual Molecular Devices SpectraMax 190 User Manual (55 pages) Microplate Spectrophotometers Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Luminescence. SpectraMax® M-Series Readers, SpectraMax® Paradigm® Systems, FlexStation® 3 Systems. 00. The Spectramax 190 has. 303 unable to cal dark current. Each sample has a discrete sample beam and reference. Check Details. Sep 19, 2023 11am PT Date Format. . spectramax quickdrop micro-volume spectrophotometer, quantitate micro-volume, quickdrop spectrophotometer specifications, nanodrop, low-volume quantitation, dna, protein, rna, oligo, spectrophotometer, uv/vis. Molecular Devices Spectramax 190 Absorbance Microplate Reader. The 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added after the incubation. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The following steps provide a quick reminder of the basic operating proce- Overview dures required to read a microplate using the SPECTRAmax 190. Description 1. Microplate Readers. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. A microplate reader for endpoint, kinetic, and spectral scan assays at UV-visible wavelengths, with temperature control, shaking capabilities, and. 2. (190 nm - 1000 nm). Ultraviolet transparent multiwell plates from Greiner Highly recommended for measurements of DNA and protein concentrations at 260 nm or 280 nm, respectively By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax® 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. It measures the optical density (OD) of samples in 96-wellSpectramax M Series, supplied by Danaher Inc, used in various techniques. Spectrophotometric assays were carried out using a SpectraMax 190 Microplate Reader (96-well flat bottom cell culture plate—Kasvi®). Protein content of supernatants was estimated using the BCA reagent. Monochromator, tunable 1. SpectraMax 190 Absorbance Microplate Reader. SPECTRAmax PLUS 384 measuring instruments pdf manual download. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. Temperature control. Near bottom. mg −1 protein using the extinction coefficient of 39. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。 The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. No accessories are needed and SoftMax® Pro Software. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After incubation, the unbound planktonic cells (120 μl) were. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Read Cuvette - For the SpectraMax Plus 384, press to initiate the sample read of the cuvette. The Spectramax Plus 384 is a UV–visible absorbance spectrophotometer used for sample analysis in microtiter wells or cuvettes. . For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Microplate Readers Promega GloMax Plate Reader Multi-Detection. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 0 nm increments * Wavelength Bandwidth 2 nm * Wavelength Accuracy < ± 2. Analysis of pro-inflammatory macrophage phenotypic. The soluble PAs were quantified by the DMACA method. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. 00 +$350. . Absorbance was read on a SpectraMax 190 plate reader (Molecular Devices, Wokingham, Berkshire, UK). Italic and boldface type are used for emphasis. See SpectraMax® Microplate Reader User Manual Download Page. min −1. 13. Open the catalog to page 1. GloMax ® Navigator System. The SpectraMax 190 reader is the tool for DNA analysis. Add to Cart Buy. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. , Tokyo, Japan) was added to each well every 24 h. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wella SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. The VersaMax and SpectraMax 190 read 96-well microplates. The stability of the luminescent signal, as. The final value was expressed as ng/g protein. This technical note is designed to provide information . Molecular Devices’ PathCheck® sensor technology. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. 9 in. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. • If SpectraMax can not communicate with a specific device, it will display a window showing which device and allow for the user to modify the communications settings or emulate the device (ifThe SpectraMax 190 reader is the tool for DNA analysis. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Performance Specifications . ; Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaksIMI detection was carried out using the Eurofins Imidacloprid ELISA kit (Eurofins Abraxis, Warminster, PA, USA) following the manufacturer’s instructions for extraction and quantification of imidacloprid residue in leaf tissue and using a spectrophotometer (SpectraMax 190; Molecular Devices, San Jose, CA, USA). For very concentrated samples, use a minimum…200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Type: Clear: Sale. The SpectraMax 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. SOFTmax PRO makes it possible to. Example: [Drawer]. Description 1. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Add to cart. softmax pro software, spectramax paradigm, spectramax m5e/m5, walk-away automation, lab automation, fda 21 cfr oart 11, glp, gmp, stakmax Created Date: 1/11/2022 12:34:59 PM. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Additional models are available. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. SpectraMax microplate reader with UV absorbance detection (Molecular Devices) UV–transparent microplates Disposable: Corning cat. Microplate spectrophotometer (54 pages) Measuring Instruments Molecular Devices FlexStation II Operator's Manual. Molecular Devices Spectramax 190 Absorbance Microplate Reader. Price: $2,200. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. 7°C (gradient heating design). overnight at room. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Standard concentrations ranged from 1x10-11 M to 1x10-6 M. 1) Turn on the power switch of the SPECTRAmax 190 (located on the back panel). Compatible automation solutions for SpectraMax Readers. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. After 1 h of incubation, the absorbance of each well was measured at A450 nm using a SpectraMax microplate reader (SpectraMax 190, Molecular Devices, USA). User Manuals, Guides and Specifications for your Molecular Devices SpectraMax 190 Measuring Instruments. Three low volume 24-well bottom slides. . Benchtop scanning fluorometer and integrated fluid transfer workstation (134 pages)SpectraMax® Plus384, 340PC384, 190, and VersaMaxTM. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . for every assay. ATP standard curves read on SpectraMax microplate readers. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. SpectraMax® 340PC384 Microplate Reader for. Molecular Devices SOFTmax PRO software is a highly integrated program that can be used to control and collect data from the SPECTRAmax 190. The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Wavelength Bandwidth. The blood was centrifuged at 3000 rpm for 15. The same was done using samples of colloidal chitin to confirm concentrations. 00. SoftMax® Pro Software User Guide — 0112-0140 Rev. Spectranet customer portalGW9662 and roscovitine were purchased from MCE (catalog nos. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Synthesis and crystallization of complex 1. Molecular Devices SpectraMax 190 Microplate Reader. Molecular Devices SpectraMax 190 and SoftMax Pro6. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Spleen Cell. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. After incubation at 37 °C for 15 min, the resulting color reaction was read at 450 nm by a microplate reader (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA, USA) within 10 min, and the final IL-1 concentration was calculated using a 4-parameter logistic curve fit. 2. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The instrument has a pathlength correction feature. Italic and boldface type are used for emphasis. Molecular Devices technical support. Manufacturer: Molecular Devices; Model: SpectraMax 190 * Wavelength Selection Monochromator, tunable 1. Wavelength Selection . SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. We have 1 Molecular Devices SpectraMax 190 manual available for free PDF download: User Manual . It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Multi-mode Microplate Readers. The activity of catalase was expressed in μmol . Ensure there are three inches between the back of the instrument and any other objects or surfaces so it has proper air flow. The mixture was shaken and incubated for 30 min at room temperature in the dark. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The VersaMax and SpectraMax 190 read 96-well microplates. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. What is the part number for the filter wheel motor? I am looking to replace mine because I suspect it is the cause of the failure• SpectraMax® 190 (Serial#-NNR-xxxxx) •ersaMax™ (Serial#-BNR-xxxxx) V • AquaMax® Microplate Washer. 05,. Capable of detecting DNA down to 25 ng per well, this model gives users the same. The multi-channel design truly mimics a dual-beam spectrophotometer. For more sample throughput, the system can The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. See More Information. HI Wendy, I dont have a lot of expertise but I'd just suggest you to check that the filter wheel isn't touching the metal chassis. Fluorescent Polarization: Solid black plates, as above. 00. Colorimetric reaction was stopped by adding 2 NH2SO4. Contact AnytimeLabTraderThe OD values of this chromogenic enzymatic reaction were read on a microplate spectrophotometer (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA) at 450 nm within 30 min after adding the stop solution. In manufacturing DNA/RNA-synthesizers for universities, hospitals and private companies all over the world, the group relies on the SpectraMax 190 Absorbance Microplate Reader. Monochromator, tunable 1. The cooled mixture was centrifuged at 4000 rpm for 10 min, and the supernatant was collected. For very concentrated samples, use a minimum0200-6262. Prior to the reading of each plate, the microplate reader was “blanked” with a. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. All the measurements were taken in triplicate and the mean values were calculated. The SpectraMax ® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on. The VersaMax and SpectraMax 190 read 96-well microplates. Examples: “Press carefully to engage,” “Do not. Usage/Application: Lab. SpectraDrop High-Throughput Screening (HTS) Kit. DNA standards of known concentrations ranging from 1. Add to Cart. 96/384-well plate formats. The absorbance was read every 30 s for a period of 3 min using a SpectraMax 190 microplate reader. Absorption was measured at 450 nm 24 h after premix added using SpectraMax 190 (Molecular Devices, LLC, Sunnyvale, CA, USA) to produce a cell growth curve. . A microplate reader for endpoint, kinetic, and spectral scan assays at UV-visible wavelengths, with temperature control, shaking capabilities, and multichannel optical design. Service Contracts, Preventative Maintenance and Performance. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Since this is an older reader, we recommend that new users choose our newer model i3 reader. 0 out of 5 $. VC was used as the control substance. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Spectramax® id3 multi-mode microplate reader, molecular devicesMolecular devices showcases spectramax I3x spectramax pdfSpectramax® i3:. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The SpectraMax 190 reader is the tool for DNA analysis. The cell pellets were lysed with 200 mmol/L NaOH, and the iron content was determined using the ferrozine assay. A bandwidth of 5 nm or less is required for accurate absorbance measurement of DNA 1. SpectraMax® Plus 384. Ultraviolet transparent multiwell plates from Greiner; Highly recommended for measurements of DNA and protein concentrations at. 1. It measures the optical density (OD) of samples in 96-well 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. ®{"general":{"total":5,"offset":0,"limit":5,"data":[{"key":"label","path":"path","name":"Name","identifier":"Identifier","wavelength-ranges":"Wavelength ranges. 0 nm increments Wavelength bandwidth 2 nm Wavelength accuracy ±1. 2 nm. ¨The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. 8 ng/mL. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date:SpectraMax 190: Located in the back of lab 302 . Three kits covering a wide range of dsDNA concentrations. The SpectraTest® ABS1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 5 µL - Detector: CCD array, 1024 element - Nguồn sáng : đèn Xenon - Nồng độ. Prepare your i3/i3x instrument for shipment. Spectramax 190 User Manual, Metrix St5484e Manual, Cm2020b Manual, Dek 5650 Manual, Manual De Sony Vegas 6, Manual For Sonicwall Tz 100, Saflok 6000 Manual one-document-company 4. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190. The VersaMax and SpectraMax 190 read 96-well microplates. SpectraMax 190 and Spect FRET fluorescence, top- le-Mode Detection Fluorescence Sir and bottom-read, cell-based assay, lal injection in both 96- and 384-well lates, excellent sensitivity, Autorinse range, du microp 0+ pre-defined protocols; lac compatible based (visible 405-750 nm)The obtained mixture was reacted in a boiling water bath for 20 min, and then, cooled down immediately. SpectraMax® Paradigm® Multi-Mode Detection Platform. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for. $3,500. Soluble PA fractions (20 μL) were mixed with 100 μL of DMACA reagent on a 96-well plate. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. Currently unavailable. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Temperature control and shaking come as standard. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. 384,340PC384,190,ABS,ABS Plus,VersaMax,FlexStation 3 0200-5060 SpectraTest FL1 FluorescenceValidation PlateMOLECULAR DEVICES VERSAMAX SPECTRAMAX 190 INSTALLATION MANUAL Pdf. Wavelength accuracy ±1. The patented multichannel optical design of the SpectraMax® 190. Patient samples were diluted 1:100, except for patients with CML for whom a dilution of 1:300 was used. Plus 384 - SpectraMax Plus 384 Microplate Reader - SpectraMax Plus 384 Absorbance Plate Reader, Molecular Devices - Each . INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). All service plan coverage will end by December 31, 2028. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Part # 0112-0085 Rev. Absorbance equation. Manufacturer: Molecular Devices; Model: SpectraMax 190; The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. spectramax plus 384 microplate reader, pathcheck sensor, spectramax plus 384 reader, softmax pro software, stakmax microplate stacker, spectratest validation package, microplate. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Our Price: $10,995. SKU: 17247 Category: Laboratory Equip. The PathCheck sensor can extend the instrument linearity by a factor of three or more. Read - For the SpectraMax 190 and SpectraMax 340PC384, press to initiate the plate read when you operate the instrument in stand alone mode. . Example: [Drawer]. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. Add to cart. 2 nm 光度量范围 0-4. . Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. ) gap between the rear of the instrument and the wall. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. T = I/I 0 and %T = 100 (T). 049 g, 0. The inter- and intra-assay CV for the SAA analysis were less than 10%. A = 2 - log 10 (%T). Brand: Molecular. 00 . SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The SpectraMax 190 has a multichannel design for 96-well Molecular Devices SpectraMax 190 Temperature range: 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices SpectraMax 190 can be used to quantify DNA and perform endpoint, kinetic, and spectral scanning analyses. SpectraMax 190 Absorbance Microplate Reader. Free shipping. For aqueous solutions, our patented* PathCheck ® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 9000-0756. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Not all manufacturers microplates are the same with regard to design, mate- rials, or conÞguration. Multimode Reader Comparison Chart. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays. SoftMax Pro Software has been integrated by many leading robotics andSPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Plus 384 Protocol (MS Word) For more. The optical density was measured at 540 nm using a SpectraMax 190 microplate reader (Molecular Devices, San Jose, CA, USA). The plate was mixed on an orbital shaker for 15 s and immediately read using a SpectraMax iD3 (Molecular Devices; San Jose, CA, USA) and an integration time of 400 ms. Our Price: $6,995. qPCR HBV DNA was extracted as. SpectraMax and VersaMax . , 0, 0. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. Time-resolved phosphorescence. 00 . The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 3) At the bottom of the strip, click on "Show. Two 4" dia, wheels are available: one which accepts six 25mm filters, and one which accepts twelve 12. The multi-channel design truly mimics a dual-beam spectrophotometer. Very small footprint suitable for tight space requirements. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-The SPECTRAmax 190 is equipped with an 8-pin DIN RS-232 serial port through which a computer can communicate with and control the instrument. The Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 00 . 0 out of 5 $ 2,499 $ 1,999. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. The PathCheck sensor can extend the instrument linearity by a factor of three or more. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. 2. Example: [Drawer]. ®User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] mg/ml, 80 μl) was incubated at 25°C in the dark for 30 min, then the absorbance recorded at 517 nm (SpectraMax 190 Microplate Reader, USA). Multimode Readers Comparison Chart. 314 low lamp level warning. SpectraMax ID5 Plate Reader (Molecular Devices) Absorbance. Cell viability was denoted with relative growth rate (RGR%), which was calculated by using. It offers accurate, precise, and flexible assay development tools, such as PathCheck Sensor Interface, PathCheck ABS1 Absorbance Plate, and SpectraTest ABS1 Absorbance Plate. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Fig 2: Enzymatic response of synthetic compounds. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Minimize sample volume while retaining sensitivity with the SpectraDrop Micro-Volume Microplate. The SPECTRAmax 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. All samples were tested in duplicate and the optical density values were read on a microplate spectrophotometer (Spectramax 190, Molecular devices Corporation, CA, USA) at 450 nm. Molecular Devices SpectraMax 190 User Manual (55 pages) Microplate Spectrophotometers. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. to 11. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Offer Ends In: 9 Days 19: 59: 40. S. The light absorbance (A) of the supernatant was measured at 450, 532, and 600 nm using an MD SpectraMax 190 full-wavelength microplate reader . For aqueous solutions, our patented* PathCheck® sensor. Add to cart. Streamline GLP/GMP compliance. 2 nm. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Exceptional performanceThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. After incubating for 10 min at room temperature, the absorbance was measured at 510 nm by SpectraMax 190 Microplate Reader (Molecular Devices, San Jose, CA). The optical density at 450 nm was measured with the SpectraMax 190 Microplate Reader (Molecular Devices). SpectraMax 190 Specifications Recommended microplate Flat-bottom microplates with isolated wells and lid AUTOMIX WITH SOFTMAX PRO Plate mixing modes Plate mixing duration Selectable: off, once prior to any reading, and once prior to and between kinetic readings Selectable: 1 to 999 seconds (three-second default) COMPATIBILITY. Three-dimensional Matrigel culture. 0 nm, and a photometric accuracy of <±0. The sensitivity of this assay was 250 pg/mL, and the intra. It measures the optical density (OD) of samples in 96-wellLow-volume, high-throughput DNA and protein detection on the SpectraMax ABS Plus Microplate Reader. The temperature in the plate chamber is ambient and isothermal. 1. The corrected absorbance is within 5% of the. 9 to 190 ng/µL were prepared in TE buffer and pipetted onto the wells (spots) of the SpectraDrop Micro-Volume. Also for: Spectramax plus. . ®System (PerkinElmer) On The SpectraMax ID5 Reader. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Briefly, the.